Influence of silver nanoparticles on fibronectin adsorption at the surface of a plasma-deposited organosilicon coatings

Laurine Martocq (1, 2) , Pascale Chevallier (2) , Morgane Laurent (2) , Gaétan Laroche (2) , Kremena Makasheva (3)

1 LAPLACE - LAboratoire PLasma et Conversion d'Energie
2 ULaval - Université Laval [Québec]
3 LAPLACE-ScIPRA - Sciences et Ingénierie des Plasmas Réactifs et des Arcs

Please select your preferred method of presentation: Free Session-Poster Introduction: At any given time, out of 100 hospitalized patients, 7 in developed and 10 in developing countries will get at least one healthcare-associated infection (HCAI). The development of HCAIs is mainly due to the material's surface colonization by microorganisms. Adherent bacteria organize themselves to form a biofilm, once the extracellular polymeric matrix made of proteins, polysaccharides, and lipids is formed [1]. The biofilm structure renders the bacteria highly resistant to antibiotics, and accordingly, there is a growing need to develop new strategies to inhibit this bacterial structuration. This project takes advantage of the known antimicrobial potential of silver nanoparticles (AgNPs) [2]. However, to achieve a long-lasting delivery of Ag + ions, fast oxidation of the AgNPs should be avoided. Protection of the AgNPs was ensured through coating with a plasma-deposited organosilicon matrix [3,4]. This study focuses on the adsorption of fibronectin (FN) at the surface of plasma-deposited organosilicon (SiOC:H) surfaces containing AgNPs, as the protein adsorption is the first step towards bacterial biofilm formation. Experimental methods: An axially-asymmetric radiofrequency capacitive plasma was used to deposit a SiOC:H matrix and AgNPs on quartz substrates (<10 Pa). The matrix was obtained using a gas mixture of hexamethyldisiloxane (HMDSO) and argon, while the AgNPs were synthesized by sputtering of the upper silver electrode in an argon plasma. Droplet of 10 µL of human FN solution in HEPES buffer at a concentration of 200 µg/mL, were deposited on the plasmadeposited SiOC:H surfaces with or without AgNPs. The adsorption lasted for 1h and was followed by 3 successive washings in water with vortex and drying. Atomic Force Microscopy (AFM), X-ray Photolectron Spectroscopy (XPS) and dynamic contact angle measurements were used to characterize the surfaces and the FN organization after adsorption.

Domaines: Sciences de l'ingénieur [physics]

Laurine Martocq, Pascale Chevallier, Morgane Laurent, Gaétan Laroche, Kremena Makasheva. Influence of silver nanoparticles on fibronectin adsorption at the surface of a plasma-deposited organosilicon coatings. 11th World Biomaterials Congress 2020, Dec 2020, Glasgow, United Kingdom. ⟨hal-03092500⟩