The involvement of calmodulin and protein kinases in the upstream of cytosolic and nucleic calcium signaling induced by hypoosmotic shock in tobacco cells

H. Nguyen (1) , François Bouteau (2, 1) , Christian Mazars (3) , M. Kuse (4) , T. Kawano (1, 5)

1 UKK - University of Kitakyushu
2 LIED (UMR_8236) - Laboratoire Interdisciplinaire des Energies de Demain
3 LRSV - Laboratoire de Recherche en Sciences Végétales
4 Konan University [Kobe, Japan]
5 Université Paris Diderot, Sorbonne Paris Cité, Paris, France

Changes in Ca2+ concentrations in cytosol ([Ca2+]C) or nucleus ([Ca2+]N) may play some vital roles in plants under hypoosmotic shock (Hypo-OS). Here, we observed that Hypo-OS induces biphasic increases in [Ca2+]C and [Ca2+]N in two tobacco cell lines (BY-2) expressing apoaequorin either in the cytosol or in the nucleus. Both [Ca2+]C and [Ca2+]N were sensitively modulated by the inhibitors of calmodulin and protein kinases, supporting the view that calmodulin suppresses the 1st peaks and and protein kinases enhance 2nd peaks in [Ca2+]C and [Ca2+]N. Data also suggested that the 1st and 2nd events depend on the internal and extracellular Ca2+ sources, respectively.

Mots clés: Calmodulin; Cytosolic calcium signaling; Hypoosmotic shock; Nucleic calcium signaling; Protein kinases; Tobacco cells

Domaines: Biologie végétale

H. Nguyen, François Bouteau, Christian Mazars, M. Kuse, T. Kawano. The involvement of calmodulin and protein kinases in the upstream of cytosolic and nucleic calcium signaling induced by hypoosmotic shock in tobacco cells. Plant Signaling and Behavior, 2018, 13 (8), pp.e1494467. ⟨10.1080/15592324.2018.1494467⟩. ⟨hal-03671726⟩