Chronic TNFalpha and cAMP pre-treatment of human adipocytes alter HSL, ATGL and perilipin to regulate basal and stimulated lipolysis.

Véronic Bézaire (1) , Aline Mairal (1) , Rodica Anesia (1) , Corinne Lefort (1) , Dominique Langin (1, 2)

1 I2MR - Institut de médecine moléculaire de Rangueil
2 Laboratoire de Biochimie [CHU Toulouse]

We examined the effects of chronic TNFalpha and dibutyryl-cAMP (Db-cAMP) pre-treatment on the lipolytic machinery of human hMADS adipocytes. TNFalpha decreased adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) protein content and triglycerides (TG)-hydrolase activity but increased basal lipolysis due to a marked reduction in perilipin (PLIN) protein content. Conversely, Db-cAMP increased ATGL and HSL protein content but prevented PLIN phosphorylation, the net result being accentuated basal lipolysis. In forskolin-stimulated conditions, TNFalpha and Db-cAMP pre-treatment decreased stimulated TG-hydrolase activity and impaired PLIN phosphorylation. Together, this resulted in a severely attenuated response to forskolin-stimulated lipolysis.

Domaines: Biochimie, Biologie Moléculaire

Véronic Bézaire, Aline Mairal, Rodica Anesia, Corinne Lefort, Dominique Langin. Chronic TNFalpha and cAMP pre-treatment of human adipocytes alter HSL, ATGL and perilipin to regulate basal and stimulated lipolysis.. FEBS Letters, 2009, 583 (18), pp.3045-9. ⟨10.1016/j.febslet.2009.08.019⟩. ⟨inserm-00419424⟩