Effect of FTY720 on apoptosis of smooth muscle cells.

Torsten Böhler (1) , J. Guillebaud (1) , Lucien Etienne (1) , Cindy Canivet (1) , Nassim Kamar (2) , Lionel Rostaing (2) , Jean-Claude Thiers (1) , Sylvain Galvani (1) , Nathalie Augé (1) , Robert Salvayre (1) , Anne Nègre-Salvayre (1) , Mogens Thomsen (1)

1 I2MR - Institut de médecine moléculaire de Rangueil
2 Département de Néphrologie et Transplantation d'organes [CHU Toulouse]

BACKGROUND: Hyperproliferation of smooth muscle cells (SMCs) plays a key role in allograft arteriosclerosis. This prompted us to investigate the effect of the novel immune modulator and synthetic sphingolipid FTY720 on apoptosis of SMCs. METHODS: Rabbit SMC cultures were treated with FTY720 and apoptosis and necrosis were detected by fluorescence microscopy. RESULTS: We investigated dose- and time-dependent effects of FTY720 and found that clinically relevant low doses of FTY720 (<1 micromol/L) did not induce apoptosis, whereas 10 micromol/L FTY720 induced apoptosis after 48 hours incubation. CONCLUSION: At doses of FTY720 used in clinics for treatment of renal allografts and multiple sclerosis. FTY720 did not induce SMC apoptosis.

Torsten Böhler, J. Guillebaud, Lucien Etienne, Cindy Canivet, Nassim Kamar, et al.. Effect of FTY720 on apoptosis of smooth muscle cells.. Transplantation Proceedings, 2007, 39 (8), pp.2624-6. ⟨10.1016/j.transproceed.2007.08.012⟩. ⟨inserm-00420472⟩