Macrophage gene expression is related to obesity and the metabolic syndrome in human subcutaneous fat as well as in visceral fat.

Eva Klimcakova (1) , Balbine Roussel (2) , Zuzana Kovacova (1) , Michaela Kovacikova (1) , Michaela Siklova-Vitkova (1) , Marion Combes (2) , Jindra Hejnova (1) , Pauline Decaunes (2) , Jean-José Maoret (2) , T. Vedral (3) , Nathalie Viguerie (2) , Virginie Bourlier (2) , Anne Bouloumié (2) , Vladimir Stich (1) , Dominique Langin (2)

1 Franco-czech Laboratory for clinical research on obesity
2 I2MC - Institut des Maladies Métaboliques et Cardiovasculaires
3 Department of surgery

AIMS/HYPOTHESIS: Our goal was to identify a set of human adipose tissue macrophage (ATM)-specific markers and investigate whether their gene expression in subcutaneous adipose tissue (SAT) as well as in visceral adipose tissue (VAT) is related to obesity and to the occurrence of the metabolic syndrome. METHODS: ATM-specific markers were identified by DNA microarray analysis of adipose tissue cell types isolated from SAT of lean and obese individuals. We then analysed gene expression of these markers by reverse transcription quantitative PCR in paired samples of SAT and VAT from 53 women stratified into four groups (lean, overweight, obese and obese with the metabolic syndrome). Anthropometric measurements, euglycaemic-hyperinsulinaemic clamp, blood analysis and computed tomography scans were performed. RESULTS: A panel of 24 genes was selected as ATM-specific markers based on overexpression in ATM compared with other adipose tissue cell types. In SAT and VAT, gene expression of ATM markers was lowest in lean and highest in the metabolic syndrome group. mRNA levels in the two fat depots were negatively correlated with glucose disposal rate and positively associated with indices of adiposity and the metabolic syndrome. CONCLUSIONS/INTERPRETATION: In humans, expression of ATM-specific genes increases with the degree of adiposity and correlates with markers of insulin resistance and the metabolic syndrome to a similar degree in SAT and in VAT.

Mots clés: Human adipose tissue; Inflammation; Macrophage; Metabolic syndrome; Obesity; Subcutaneous fat; Visceral fat

Domaines: Biochimie, Biologie Moléculaire

Eva Klimcakova, Balbine Roussel, Zuzana Kovacova, Michaela Kovacikova, Michaela Siklova-Vitkova, et al.. Macrophage gene expression is related to obesity and the metabolic syndrome in human subcutaneous fat as well as in visceral fat.. Diabetologia, 2011, 54 (4), pp.876-87. ⟨10.1007/s00125-010-2014-3⟩. ⟨inserm-00617667⟩