Involvement of tissue bacteria in the onset of diabetes in humans: evidence for a concept.

Jacques Amar (1, 2) , Matteo Serino (3) , Cécile Lange (4) , Chantal Chabo (3) , Jason Iacovoni (3) , Stanislas Mondot (5) , Patricia Lepage (5) , Christophe C. Klopp (6) , Jérôme J. Mariette (6) , Olivier Bouchez (7) , Laurence Perez (3) , Michael Courtney (8) , Michel Marre (9, 10) , Pascale Klopp (3) , Olivier Lantieri (11) , Joël Doré (5) , Marie-Aline Charles (4) , Beverley Balkau (4) , Rémy Burcelin (3)

1 LEASP - Epidémiologie et analyses en santé publique : risques, maladies chroniques et handicaps
2 HTA et thérapeutique - Service d'hypertension artérielle et thérapeutique [CHU Toulouse]
3 I2MC - Institut des Maladies Métaboliques et Cardiovasculaires
4 CESP - Centre de recherche en épidémiologie et santé des populations
5 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
6 UBIA - Unité de Biométrie et Intelligence Artificielle (ancêtre de MIAT)
7 GENOTOUL - Génopole Toulouse Midi-Pyrénées [Auzeville]
8 Vaiomer
9 (U 695) - Déterminants génétiques du diabète de type 2 et de ses complications vasculaires
10 Service d'endocrinologie, diabétologie et nutrition [CHU Bichat]
11 IRSA - Institut inter-Régional pour la SAnté

AIMS/HYPOTHESIS: Evidence suggests that bacterial components in blood could play an early role in events leading to diabetes. To test this hypothesis, we studied the capacity of a broadly specific bacterial marker (16S rDNA) to predict the onset of diabetes and obesity in a general population. METHODS: Data from an Epidemiological Study on the Insulin Resistance Syndrome (D.E.S.I.R.) is a longitudinal study with the primary aim of describing the history of the metabolic syndrome. The 16S rDNA concentration was measured in blood at baseline and its relationship with incident diabetes and obesity over 9 years of follow-up was assessed. In addition, in a nested case-control study in which participants later developed diabetes, bacterial phylotypes present in blood were identified by pyrosequencing of the overall 16S rDNA gene content. RESULTS: We analysed 3,280 participants without diabetes or obesity at baseline. The 16S rDNA concentration was higher in those destined to have diabetes. No difference was observed regarding obesity. However, the 16S rDNA concentration was higher in those who had abdominal adiposity at the end of follow-up. The adjusted OR (95% CIs) for incident diabetes and for abdominal adiposity were 1.35 (1.11, 1.60), p = 0.002 and 1.18 (1.03, 1.34), p = 0.01, respectively. Moreover, pyrosequencing analyses showed that participants destined to have diabetes and the controls shared a core blood microbiota, mostly composed of the Proteobacteria phylum (85-90%). CONCLUSIONS/INTERPRETATION: 16S rDNA was shown to be an independent marker of the risk of diabetes. These findings are evidence for the concept that tissue bacteria are involved in the onset of diabetes in humans.

Mots clés: abdominal adiposity; obesity; metabolism; endocrinology; 16s rdna gene

Mots clés: microbiota

Domaines: Sciences du Vivant [q-bio]; Mathématiques [math]; Informatique [cs]

Jacques Amar, Matteo Serino, Cécile Lange, Chantal Chabo, Jason Iacovoni, et al.. Involvement of tissue bacteria in the onset of diabetes in humans: evidence for a concept.. Diabetologia, 2011, 54 (12), pp.3055-61. ⟨10.1007/s00125-011-2329-8⟩. ⟨inserm-00801269⟩