Effect of low dose of fumonisins on pig health: Immune status, intestinal microbiota and sensitivity to Salmonella

Christine Burel (1) , Maël Tanguy (1) , Philippe Guerre (2) , Eric Boilletot (1) , Roland Cariolet (1) , Marilyne Queguiner (1) , Gilbert Postollec (1) , Philippe Pinton (3) , Gilles Salvat (1) , Isabelle P. Oswald (3) , Philippe Fravalo (1)

1 ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
2 ENVT - Ecole Nationale Vétérinaire de Toulouse
3 ToxAlim-BioToMyc - Biosynthèse & Toxicité des Mycotoxines

The objective of this study was to measure the effects of chronic exposure to fumonisins via the ingestion of feed containing naturally contaminated corn in growing pigs infected or not with Salmonella spp. This exposure to a moderate dietary concentration of fumonisins (11.8 ppm) was sufficient to induce a biological effect in pigs (Sa/So ratio), but no mortality or pathology was observed over 63 days of exposure. No mortality or related clinical signs, even in cases of inoculation with Salmonella (5 x 10(4) CFU), were observed either. Fumonisins, at these concentrations, did not affect the ability of lymphocytes to proliferate in the presence of mitogens, but after seven days post-inoculation they led to inhibition of the ability of specific Salmonella lymphocytes to proliferate following exposure to a specific Salmonella antigen. However, the ingestion of fumonisins had no impact on Salmonella translocation or seroconversion in inoculated pigs. The inoculation of Salmonella did not affect faecal microbiota profiles, but exposure to moderate concentrations of fumonisins transiently affected the digestive microbiota balance. In cases of co-infection with fumonisins and Salmonella, the microbiota profiles were rapidly and clearly modified as early as 48 h post-Salmonella inoculation. Therefore under these experimental conditions, exposure to an average concentration of fumonisins in naturally contaminated feed had no effect on pig health but did affect the digestive microbiota balance, with Salmonella exposure amplifying this phenomenon.

Mots clés: pig; fumonisins; growth; health; co-contamination; CULTURE MATERIAL; WEANED PIGLETS; FUSARIUM-MONILIFORME; ESCHERICHIA-COLI; B-1 ALTERS; SWINE; DEOXYNIVALENOL; B1

Mots clés: mycotoxins; immune status; intestinal microbiota; Salmonella; PASTEURELLA-MULTOCIDA; EXPOSURE

Domaines: Sciences du Vivant [q-bio]

Christine Burel, Maël Tanguy, Philippe Guerre, Eric Boilletot, Roland Cariolet, et al.. Effect of low dose of fumonisins on pig health: Immune status, intestinal microbiota and sensitivity to Salmonella. Toxins, 2013, 5 (4), pp.841 - 864. ⟨10.3390/toxins5040841⟩. ⟨hal-02651583⟩