MISFET-based biosensing interface for neurons guided growth and neuronal electrical activities recording

Florian Larramendy 1 Amel Bendali 2 Marie-Charline Blatché 3 Fabrice Mathieu 3 Serge Picaud 2 Pierre Temple-Boyer 4 Liviu Nicu 1
1 LAAS-NBS - Équipe NanoBioSystèmes
LAAS - Laboratoire d'analyse et d'architecture des systèmes
3 LAAS-I2C - Service Instrumentation Conception Caractérisation
LAAS - Laboratoire d'analyse et d'architecture des systèmes
4 LAAS-MICA - Équipe MICrosystèmes d'Analyse
LAAS - Laboratoire d'analyse et d'architecture des systèmes
Abstract : A hybrid circuit of a transistor-based chip was implemented and characterized for the neuronal electrical activity recording. The integration of microfluidic architectures was developed to control precisely neurites outgrowth and form topologically defined and stable neural networks. Individual neural cells from rat retinae and Lymnaea stagnalis snails were immobilized on gates regions of Metal Insulator Semiconductor Field Effect Transistors (MISFET). Neuronal orientation was achieved in both cases but neuronal action potentials were only recorded in the Lymnaea stagnalis case. They were successfully triggered and inhibited by implementing a picrotoxin-GABA – picrotoxin injection protocol, exhibiting a direct influence of picrotoxin on the "spike type" action potential waveform. The implementation of the whole process of neuronal culture and subsequent activity monitoring constitutes a proof-of-principle experiment for the development of neuroelectronic systems for signal processing studies adapted to low-density neuronal cultures.
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Florian Larramendy, Amel Bendali, Marie-Charline Blatché, Fabrice Mathieu, Serge Picaud, et al.. MISFET-based biosensing interface for neurons guided growth and neuronal electrical activities recording. Sensors and Actuators B: Chemical, Elsevier, 2014, 203, pp.375 - 381. ⟨10.1016/j.snb.2014.06.106⟩. ⟨hal-01504965⟩

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