Characterization of glucan-producing Leuconostoc strains isolated from sourdough
Résumé
Sourdough was previously demonstrated to be a fruitful biotope for isolation of lactic acid bacteria producing exopolysaccharides and more accurately diverse glycan polymers which have interesting applications as texturing agents or prebiotics. Characterization of polymers by (1)H and (13)C NMR spectroscopy analysis demonstrated that these strains could synthesize glucans of high structural variety and containing different amounts of alpha-(1 -> 2), alpha-(1 -> 3) and alpha-(1 -> 6) linkages. In this study, fifteen glucan-producing Leuconostoc mesenteroides and L citreum strains from sourdoughs were characterized according to carbohydrate fermentation, rep-PCR fingerprinting using (GTG)(5) primers and glycansucrase activity (soluble or cell-associated). Enzyme characterization using SOS-PAGE and in situ polymer production after incubation with sucrose correlated with synthesis of classical or alpha-(1 -> 2) branched dextrans, alternan and levan. In addition, the presence of genes coding for alternansucrase was detected by PCR and partially characterized by sequence analysis. We thus provide new information on the biodiversity of glucan production by sourdough Leuconostoc strains. (C) 2010 Elsevier B.V. All rights reserved.