Hydrolysis of concentrated lignocellulose suspensions with a cumulative feeding strategy based on a critical concentration: From laboratory to pilot scale

Tien-Cuong Nguyen 1, 2, 3 Dominique Anne-Archard 4, 3 Véronique Coma 5 Frédérique Pichavant 5 Xavier Cameleyre 1, 2, 3 Eric Lombard 1, 2, 3 Kim Anh To 6 Luc Fillaudeau 1, 2, 3
1 LISBP - Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2 Université Fédérale Toulouse Midi-Pyrénées
3 FERMAT - Fédération de Recherche Fluides, Energie, Réacteurs, Matériaux et Transferts
4 IMFT - Institut de mécanique des fluides de Toulouse
5 LCPO - Laboratoire de Chimie des Polymères Organiques
6 School of Biotechnology and Food Technology
Abstract : In the context of biofuels and chemical production of petroleum substitutes from renewable carbon, bioconversion of lignocellulose biomasses is currently a major challenge. The limited knowledge of liquefaction and saccharification mechanisms stands as the main factor which penalizes bio-refinery progress. The objective of the present work was to increase the substrate concentration during hydrolysis in controlling transfer limitations by a specific strategy of substrate feeding. This concept was studied at laboratory scale and transposed into pilot scale. In-situ and ex-situ rheometry, morpho-granulometry and biochemical measurements were used to investigate transfer limitations. In a preliminary step (lab scale), rheological behaviour was modelled and a critical concentration (Ccrit ) inducing a sharp increase of viscosity was identified for Whatman paper (WP, 35 gdm.L-1) and paper pulp (PP, 31 gdm.L-1). Analysing hydrolysis experiments leads to assume an optimal feed rate Q* linked to this critical concentration. In a first step, cumulative feeding strategies (up to 10%w/w) were conducted for WP and PP in 2L reactor with different ratios Q/Q*. A cumulative feeding substrate strategy allows to considerably reduce the transfer limitations linked to high concentrations and to control the glucose production kinetics. In the second step, the enzymatic hydrolysis (up to 20%w/w) was investigated in a 20L reactor coupled with an extruder (substrate feeding). This transposition will be discussed regarding the biochemical kinetics and physical limitations.
Keywords : Bioprocess Feed Multi-scale conception Rheology
Document type :
Poster communications
Complete list of metadatas
https://hal.archives-ouvertes.fr/hal-01886480
Contributor : Archive Ouverte Prodinra <>
Submitted on : Tuesday, October 2, 2018 - 8:15:12 PM
Last modification on : Thursday, November 21, 2019 - 9:58:50 AM

Identifiers

  • HAL Id : hal-01886480, version 1
  • PRODINRA : 435114

Collections

CNRS | INRA | INC-CNRS | INSA-TOULOUSE | INSA-GROUPE | UNIV-TLSE3 | TBI

Citation

Tien-Cuong Nguyen, Dominique Anne-Archard, Véronique Coma, Frédérique Pichavant, Xavier Cameleyre, et al.. Hydrolysis of concentrated lignocellulose suspensions with a cumulative feeding strategy based on a critical concentration: From laboratory to pilot scale. 3. European Congress of Applied Biotechnology (ECAB 3), Sep 2015, Nice, France. 1736 p., 2015. ⟨hal-01886480⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

24

CONTACT

scd.ao@univ-tlse3.fr