Spatial localization and binding of the probiotic Lactobacillus farciminis to the rat Intestinal mucosa: Influence of chronic Stress

Stéphanie da Silva (1, 2) , Catherine Robbe Masselot (3) , Arthur Raymond (1) , Myriam Mercade Loubière (1) , Christel Cartier (4) , Bélinda Ringot (5, 3) , Renaud Léonard (5, 3) , Isabelle Fourquaux (6) , Afifa Ait-Belgnaoui (2, 7) , Pascal Loubière (1) , Vassilia Theodorou (8) , Muriel Mercier-Bonin (1, 8)

1 LISBP - Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2 ToxAlim - ToxAlim
3 Université Lille Nord de France (COMUE)
4 ToxAlim-ENTeRisk - Endocrinologie & Toxicologie de la Barrière Intestinale
5 Université de Lille, Sciences et Technologies
6 CMEAB - Centre de Microscopie Électronique Appliquée à la Biologie
7 Lallemand S.A.S.
8 ToxAlim-NGN - Neuro-Gastroentérologie & Nutrition

The present study aimed at detecting the exogenously applied probiotic Lactobacillus farciminis in rats, after exposure to IBS-like chronic stress, based on 4-day Water Avoidance Stress (WAS). The presence of L. farciminis in both ileal and colonic mucosal tissues was demonstrated by FISH and qPCR, with ileum as the preferential niche, as for the SFB population. A different spatial distribution of the probiotic was observed: in the ileum, bacteria were organized in micro-colonies more or less close to the epithelium whereas, in the colon, they were mainly visualized far away from the epithelium. When rats were submitted to WAS, the L. farciminis population substantially decreased in both intestinal regions, due to a stress-induced increase in colonic motility and defecation, rather than a modification of bacterial binding to the intestinal mucin Muc2.

Domaines: Sciences du Vivant [q-bio]; Ingénierie des aliments; Génie des procédés

Stéphanie da Silva, Catherine Robbe Masselot, Arthur Raymond, Myriam Mercade Loubière, Christel Cartier, et al.. Spatial localization and binding of the probiotic Lactobacillus farciminis to the rat Intestinal mucosa: Influence of chronic Stress. PLoS ONE, 2015, 10 (9), ⟨10.1371/journal.pone.0136048⟩. ⟨hal-02637027⟩